Module 11: Figure purinergic chemotaxis
Disruption of chemotaxis by deletion of P2Y2 and A3 purinergic receptors.
The first panel shows how wild-type (WT) mouse neutrophils migrate in a directed way towards a source of fMet-Leu-Phe (fMLP). The middle panel shows that neutrophils lacking the adenosine A3 receptor (A3−/−) retain a sense of direction, but their motility is severely impaired. On the other hand, deletion of the P2Y2 receptor (P2Y2−/−) does not affect motility, but there is a loss of gradient sensing. Reproduced from Chen, Y., Corriden, R., Inoue, Y., Yip, L., Hashiguchi, N., Zinkernagel, A., Nizet, V. Insel, P.A., and Junger, W.G. (2006) ATP release guides neutrophil chemotaxis via P2Y2 and A3 receptors. Science 314:1792–1795; with permission from AAAS; http://www.sciencemag.org; see Chen et al. 2006.