Module 11: Figure SHIP1 and PIP3 polarity
Src homology 2 (SH2) domain-containing inositol 5-phosphatase 1 (SHIP1) controls neutrophil polarity and motility.
A. Wild-type (WT) and Src homology 2 (SH2) domain-containing inositol 5-phosphatase 1 (SHIP1)−/− neutrophils were placed in an EZ-chamber and their positions were monitored at 30 s intervals as they responded to a gradient of fMet-Leu-Phe (fMLP). The WT cells moved in a relatively straight line up the gradient, whereas the SHIP1−/− cells moved more slowly. B. When viewed at higher magnification, the WT cells were rounded, but assumed an elongated shape with a pseudopod at one end when placed in a fMLP gradient. By contrast, the SHIP1−/− neutrophils remained rounded with pseudopodia appearing all round the cell. If recorded over a longer period, these cells did develop a partial polarity and there was some motion up the gradient (see panel C). Reproduced by permission from Macmillan Publishers Ltd: Nishio, M., Watanabe, K., Sasaki, J., Taya, C., Takasuga, S., Iizuka, R., Balla, T., Yamazaki, M., Watanabe, H., Itoh, R., Kuroda, S., Horie, Y., Forster, I., Mak, T.W., Yonekawa, H., Penninger, J.M., Kanaho, Y., Suzuki, A. and Sasaki, T. (2007) Control of cell polarity and motility by the PtdIns(3,4,5)P3 phosphatase SHIP1. Nat. Cell Biol. 9:36–44. Copyright (2007); http://www.nature.com/ncb; see Nishio et al. 2007.