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Module 10: Figure ER Ca2+ loading

Activity-dependent loading of the endoplasmic reticulum (ER) of a hippocampal neuron.

Following stimulation of a hippocampal neuron, there was a marked increase in the Ca2+ content of the endoplasmic reticulum (ER) (solid bars), with little or no change in the cytoplasm (open bars) or mitochondria (dotted bars). The uptake of Ca2+ was graded in that it was larger after four trains compared with one train of action potentials. Reproduced, with permission, from Pozzo-Miller, L.D., Pivovarova, N.B., Leapman, R.D., Buchanan, R.A., Reese, T.R. and Andrews, S.B. (1997) Activity-dependent calcium sequestration in dendrites of hippocampal neurons in brain slices. J. Neurosci. 17:8729–8738. Copyright (1997) by the Society of Neuroscience; see Pozzo-Miller et al. 1997.