High resolution

Module 8: Figure embryonic Ca2+ wave

Pan-embryonic intercellular wave in the blastoderm margin of a zebrafish embryo.

A zebrafish embryo loaded with aequorin reveals the presence of an intercellular wave that travels around the blastoderm margin in a clockwise direction at a rate of about 5 μm/s. The first gastrulation wave began at about 65% epiboly (see timings on Module 8: Figure embryonic Ca2+ transients), and subsequent waves were repeated at about 10 min intervals for about 2 h until blastopore closure. Reproduced by permission from Macmillan Publishers Ltd: Nat. Rev. Mol. Cell Biol., Webb, S.E. and Miller, A.L. (2003) Calcium signalling during embryonic development. 4:539–551. Copyright (2003); http://www.nature.com/nrm; see Webb and Miller 2003.