Module 8: Figure embryonic Ca2+ transients
Pattern of Ca2+ transients in a developing zebrafish embryo.
Aequorin was used to record Ca2+ in the transparent zebrafish embryo over a period of 10 h, during which most of the developmental events occur. Many of the major Ca2+ transients have a precise spatial organization (see the text for details), indicating that they may have a role to play in driving specific developmental processes. Some of the transients that occur at 75% epiboly occur as intercellular Ca2+ waves that sweep around the blastoderm margin (see Module 8: Figure embryonic Ca2+ wave). Reproduced from Gilland, E., Miller, A.L., Karplus, E., Baker, R. and Webb, S.E. (1999) Imaging of multicellular large-scale rhythmic calcium waves during zebrafish gastrulation. Proc. Natl. Acad. Sci. U.S.A. 96:157–161. Copyright (1999) National Academy of Sciences, U.S.A.; see Gilland et al. 1999.