Module 8: Figure ICSI-induced Ca2+ oscillations
Ca2+ oscillations following artificial insemination by intracytoplasmic spermatozoa injection (ICSI).
A. A single spermatozoon was injected into a mouse oocyte at time zero. After recovery from the initial injection transient, there was a latency of 10 min before the onset of the first transient (transient), followed by a series of transients similar to those observed during normal fertilization, which lasted for 6 h as shown by the continuation of the recording shown in traces B and C. Reproduced, with permission, from Nakano, Y., Shirakawa, H., Mitsuhashi, N., Kuwabara, Y. and Miyazaki, S., Spatiotemporal dynamics of intracellular calcium in the mouse egg injected with a spermatozoon, Mol. Hum. Reprod., 2001, vol. 3, pp. 1087–1093; Copyright 2001 European Society of Human Reproduction and Embryology; http://humrep.oxfordjournals.org/; see Nakano et al. 2001.