Module 7: Figure sparks and blinks
Simultaneous measurement of sparks and blinks in a cardiac ventricular cell.
The level of Ca2+ with the cytoplasm and lumen of the sarcoplamsic reticulum (SR) was measured simultaneously using Rhod-2 and Fluo-5N respectively. A. In the scans in the upper two panels, Rhod-2 detected two sparks that were associated with two blinks detected with Fluo-5N. The traces below show the time course for the sparks (black) and the blinks (blue). B. These two traces illustrate that the two events occur simultaneously. Reproduced from Brochet, D.X.P., Yang, D., Di Maio, A.D., Lederer, W.J., Franzini-Armstrong, C. and Cheng, H. (2005) Ca2+ blinks: rapid nanoscopic store calcium signaling. Proc. Natl. Acad. Sci. U.S.A. 102:3099–3104. Copyright (2005) National Academy of Sciences, U.S.A; see Brochet et al. 2005.