Module 7: Figure ICC network synchrony
Gap junctions maintain ICC network slow wave synchrony.
Recordings of intracellular Ca2+ were made from cells (coloured rings) that make up the ICC network in mouse ileum. A, Control cells. B, Cells treated with β-glycyrrhetinic acid (10 μM) to disrupt the gap junctions. C. A spatiotemporal plot obtained by averaging fluorescence intensity down a column of pixels in the Y axis revealed a synchronous elevation of Ca2+ throughout the network. D, After disruption of gap junctions, individual cells fired independently of each other. E and F, These recordings revealed that the Ca2+ transients were tightly synchronized (E) whereas this synchronization disappeared when electrical coupling through the gap junctions was inhibited (F). Reproduced from Park et al. (2006).