High resolution

Module 7: Figure astrocyte Ca2+ oscillations

Ca2+ oscillations in astrocytes located within the CA1 region of the hippocampus.

Astrocytes loaded with Calcium Green acetoxymethyl ester (AM) were found to display periodic Ca2+ transients. Cells 1 and 3 (as labelled in the figure) displayed fairly regular oscillations, whereas cell 4 was quiescent throughout the recording period. Note that all of the cells displayed a large Ca2+ transient when the preparation was treated with the metabotropic glutamate receptor agonist 1-aminocyclopentane-trans-1,3-dicarboxylic acid (tACPD). Reproduced from Nett, W.J., Oloff, S.H. and McCarthy, K.D. (2002) Hippocampal astrocytes in situ exhibit calcium oscillations that occur independent of neuronal activity. J. Neurophysiol. 87:528–537, with the permission of the American Physiological Society; see Nett et al. 2002.